
Description
This user manual on the PlutoF platform focuses on the management and mobilisation of environmental DNA (eDNA) data. It outlines the entire biological data lifecycle: from project creation and permission configuration to field collection using the PlutoF GO mobile app, with QR barcode support. It details: sample management on the PlutoF workbench; data submission to global databases such as ENA (European Nucleotide Archive) and BioSamples; the import of representative sequences (OTU/ASV); and association with UNITE SH taxonomic identifiers via analysis tools. Finally, it covers the official publication of the dataset on GBIF (Global Biodiversity Information Facility) in compliance with FAIR principles, resulting in a citable DOI.
Learning Objectives
By the end of this resource, you will learn how to:
- Discuss key terminology and explain the end-to-end workflow for eDNA data management, understanding the importance of global standards and the role of PlutoF;
- Explain usage of the PlutoF workbench and the PlutoF GO mobile app to create projects, defining identifier formats, printing QR labels, recording samples in the field, and importing CSV template files;
- Examine, compare, and verify bioinformatics analysis results assessing dataset quality and compliance with ENA and GBIF archiving requirements.
- Assemble and publish new datasets, complete with metadata, references, and identifiers, on GBIF platforms, generating Darwin Core Archives (DwCA) and obtaining a DOI to ensure research citability.
Target Audience
This guide is aimed at researchers, scientific collection curators, data managers, students, and citizen scientists working in the environmental and natural sciences, focusing on the management, standardization, and archiving of biological and molecular data.




































































































